vector backbone pcdna3 1 myc his a Search Results


pcdna3.1/myc-his vector  (Thermo Fisher)
90
Thermo Fisher pcdna3.1/myc-his vector
Pcdna3.1/Myc His Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector backbone pcdna3 1 myc his a  (New England Biolabs)
99
New England Biolabs vector backbone pcdna3 1 myc his a
Vector Backbone Pcdna3 1 Myc His A, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3.1/myc-his(−)a  (Thermo Fisher)
90
Thermo Fisher pcdna3.1/myc-his(−)a
Pcdna3.1/Myc His(−)A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3.1/v5-his a  (Thermo Fisher)
90
Thermo Fisher pcdna3.1/v5-his a
Pcdna3.1/V5 His A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pcdna3.1/v5-his a - by Bioz Stars, 2026-03
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pcdna3.1(+)myc-his  (GenScript corporation)
90
GenScript corporation pcdna3.1(+)myc-his
Pcdna3.1(+)Myc His, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3.1(+)myc-his/product/GenScript corporation
Average 90 stars, based on 1 article reviews
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pcdna3.1/v5-his/a  (Thermo Fisher)
90
Thermo Fisher pcdna3.1/v5-his/a
Pcdna3.1/V5 His/A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3.1-hisa vector  (Thermo Fisher)
90
Thermo Fisher pcdna3.1-hisa vector
Pcdna3.1 Hisa Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tlr9 overexpression vector pcdna3 1 hisa  (Addgene inc)
93
Addgene inc tlr9 overexpression vector pcdna3 1 hisa
Effect of LPS on cytotoxicity and <t>TLR9</t> expression in A549 and Beas-2B cells. The viability of A549 (A) and Beas-2B (B) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. TLR9 protein expression in A549 (C) and Beas-2B (D) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. Data represent mean ± SD (n = 3). ***P<0.001 compared to 0 ng/ml.
Tlr9 Overexpression Vector Pcdna3 1 Hisa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3.1/hisa mammalian expression vectors  (Qiagen)
90
Qiagen pcdna3.1/hisa mammalian expression vectors
Effect of LPS on cytotoxicity and <t>TLR9</t> expression in A549 and Beas-2B cells. The viability of A549 (A) and Beas-2B (B) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. TLR9 protein expression in A549 (C) and Beas-2B (D) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. Data represent mean ± SD (n = 3). ***P<0.001 compared to 0 ng/ml.
Pcdna3.1/Hisa Mammalian Expression Vectors, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 1 myc hisa expression vector  (Thermo Fisher)
86
Thermo Fisher pcdna3 1 myc hisa expression vector
Effect of LPS on cytotoxicity and <t>TLR9</t> expression in A549 and Beas-2B cells. The viability of A549 (A) and Beas-2B (B) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. TLR9 protein expression in A549 (C) and Beas-2B (D) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. Data represent mean ± SD (n = 3). ***P<0.001 compared to 0 ng/ml.
Pcdna3 1 Myc Hisa Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 1 myc hisa expression vector/product/Thermo Fisher
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pcdna3.1a/myc-his vector backbone  (Thermo Fisher)
90
Thermo Fisher pcdna3.1a/myc-his vector backbone
Effect of LPS on cytotoxicity and <t>TLR9</t> expression in A549 and Beas-2B cells. The viability of A549 (A) and Beas-2B (B) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. TLR9 protein expression in A549 (C) and Beas-2B (D) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. Data represent mean ± SD (n = 3). ***P<0.001 compared to 0 ng/ml.
Pcdna3.1a/Myc His Vector Backbone, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3.1a/myc-his vector backbone/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
pcdna3.1a/myc-his vector backbone - by Bioz Stars, 2026-03
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pcdna3.1/hisa vector  (Thermo Fisher)
90
Thermo Fisher pcdna3.1/hisa vector
Effect of LPS on cytotoxicity and <t>TLR9</t> expression in A549 and Beas-2B cells. The viability of A549 (A) and Beas-2B (B) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. TLR9 protein expression in A549 (C) and Beas-2B (D) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. Data represent mean ± SD (n = 3). ***P<0.001 compared to 0 ng/ml.
Pcdna3.1/Hisa Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3.1/hisa vector/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
pcdna3.1/hisa vector - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Effect of LPS on cytotoxicity and TLR9 expression in A549 and Beas-2B cells. The viability of A549 (A) and Beas-2B (B) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. TLR9 protein expression in A549 (C) and Beas-2B (D) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. Data represent mean ± SD (n = 3). ***P<0.001 compared to 0 ng/ml.

Journal: Physiological Research

Article Title: Protection of Toll-Like Receptor 9 Against Lipopolysaccharide-Induced Inflammation and Oxidative Stress of Pulmonary Epithelial Cells via MyD88-Mediated Pathways

doi: 10.33549/physiolres.934741

Figure Lengend Snippet: Effect of LPS on cytotoxicity and TLR9 expression in A549 and Beas-2B cells. The viability of A549 (A) and Beas-2B (B) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. TLR9 protein expression in A549 (C) and Beas-2B (D) cells in response to treatment with different concentrations of LPS at 24, 48, and 72 h. Data represent mean ± SD (n = 3). ***P<0.001 compared to 0 ng/ml.

Article Snippet: Plasmid and transfection The TLR9 overexpression vector pcDNA3.1 HISA (TLR9 sequence: F: 5′-CGGAATTCCGATGGGTTTCTGCCGCAGC-3′, R: 5′-CCCTCGAGGGCTATT-CGGCCGTGGGTCCC-3′) and interference vector pSIREN (shRNA1 sequence: 5′-CCTACAACCGCATCGTCAAAC-3′, shRNA2 sequence: 5′-GCTGCCCAAATCCCTCATATC-3′, shRNA3 sequence: 5′-GCTAGACCTGTCCCACAATAA-3′) were purchased from Addgene (Cambridge, MA, USA).

Techniques: Expressing

Detection of TLR9 protein expression after transfection. TLR9 protein expression in A549 (A) and Beas-2B (B) cells. Data represent mean ± SD (n = 3). ***P < 0.001 compared to CON, ###P < 0.001 compared to OV-TLR9-EV, &&&P < 0.001 compared to sh-TLR9-EV.

Journal: Physiological Research

Article Title: Protection of Toll-Like Receptor 9 Against Lipopolysaccharide-Induced Inflammation and Oxidative Stress of Pulmonary Epithelial Cells via MyD88-Mediated Pathways

doi: 10.33549/physiolres.934741

Figure Lengend Snippet: Detection of TLR9 protein expression after transfection. TLR9 protein expression in A549 (A) and Beas-2B (B) cells. Data represent mean ± SD (n = 3). ***P < 0.001 compared to CON, ###P < 0.001 compared to OV-TLR9-EV, &&&P < 0.001 compared to sh-TLR9-EV.

Article Snippet: Plasmid and transfection The TLR9 overexpression vector pcDNA3.1 HISA (TLR9 sequence: F: 5′-CGGAATTCCGATGGGTTTCTGCCGCAGC-3′, R: 5′-CCCTCGAGGGCTATT-CGGCCGTGGGTCCC-3′) and interference vector pSIREN (shRNA1 sequence: 5′-CCTACAACCGCATCGTCAAAC-3′, shRNA2 sequence: 5′-GCTGCCCAAATCCCTCATATC-3′, shRNA3 sequence: 5′-GCTAGACCTGTCCCACAATAA-3′) were purchased from Addgene (Cambridge, MA, USA).

Techniques: Expressing, Transfection

Effect of TLR9 on the expression of cytokines in supernatants of LPS-challenged A549 and Beas-2B cells. The levels of IL-1β, IL-6, and TNF-α in A549 (A) and Beas-2B (B) cells. The levels of IFN-γ, IFN-α, and IFN-β in A549 (C) and Beas-2B (D) cells. The levels of MCP-1 and MCP-5 in A549 (E) and Beas-2B (F). Data represent mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001

Journal: Physiological Research

Article Title: Protection of Toll-Like Receptor 9 Against Lipopolysaccharide-Induced Inflammation and Oxidative Stress of Pulmonary Epithelial Cells via MyD88-Mediated Pathways

doi: 10.33549/physiolres.934741

Figure Lengend Snippet: Effect of TLR9 on the expression of cytokines in supernatants of LPS-challenged A549 and Beas-2B cells. The levels of IL-1β, IL-6, and TNF-α in A549 (A) and Beas-2B (B) cells. The levels of IFN-γ, IFN-α, and IFN-β in A549 (C) and Beas-2B (D) cells. The levels of MCP-1 and MCP-5 in A549 (E) and Beas-2B (F). Data represent mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: Plasmid and transfection The TLR9 overexpression vector pcDNA3.1 HISA (TLR9 sequence: F: 5′-CGGAATTCCGATGGGTTTCTGCCGCAGC-3′, R: 5′-CCCTCGAGGGCTATT-CGGCCGTGGGTCCC-3′) and interference vector pSIREN (shRNA1 sequence: 5′-CCTACAACCGCATCGTCAAAC-3′, shRNA2 sequence: 5′-GCTGCCCAAATCCCTCATATC-3′, shRNA3 sequence: 5′-GCTAGACCTGTCCCACAATAA-3′) were purchased from Addgene (Cambridge, MA, USA).

Techniques: Expressing

Effect of TLR9 on iNOS, eNOS, NO, and ROS production in supernatants of LPS-stimulated A549 and Beas-2B cells. iNOS level in A549 (A) and Beas-2B (B) cells. eNOS level in A549 (C) and Beas-2B (D) cells. NO generation in A549 (E) and Beas-2B (F) cells. The ROS level in A549 (G) and Beas-2B (H) cells. Data represent mean ± SD (n = 3). **P < 0.01, ***P < 0.001.

Journal: Physiological Research

Article Title: Protection of Toll-Like Receptor 9 Against Lipopolysaccharide-Induced Inflammation and Oxidative Stress of Pulmonary Epithelial Cells via MyD88-Mediated Pathways

doi: 10.33549/physiolres.934741

Figure Lengend Snippet: Effect of TLR9 on iNOS, eNOS, NO, and ROS production in supernatants of LPS-stimulated A549 and Beas-2B cells. iNOS level in A549 (A) and Beas-2B (B) cells. eNOS level in A549 (C) and Beas-2B (D) cells. NO generation in A549 (E) and Beas-2B (F) cells. The ROS level in A549 (G) and Beas-2B (H) cells. Data represent mean ± SD (n = 3). **P < 0.01, ***P < 0.001.

Article Snippet: Plasmid and transfection The TLR9 overexpression vector pcDNA3.1 HISA (TLR9 sequence: F: 5′-CGGAATTCCGATGGGTTTCTGCCGCAGC-3′, R: 5′-CCCTCGAGGGCTATT-CGGCCGTGGGTCCC-3′) and interference vector pSIREN (shRNA1 sequence: 5′-CCTACAACCGCATCGTCAAAC-3′, shRNA2 sequence: 5′-GCTGCCCAAATCCCTCATATC-3′, shRNA3 sequence: 5′-GCTAGACCTGTCCCACAATAA-3′) were purchased from Addgene (Cambridge, MA, USA).

Techniques:

Effect of TLR9 on LPS-induced apoptosis in A549 and Beas-2B cells. Flow cytometry was used to measure the percentage of apoptotic A549 (A) and Beas-2B (B) cells. Expression of apoptosis-related proteins caspase 8 and Bcl-2 in A549 (C) and Beas-2B (D) cells. Data represent mean ± SD (n = 3). ***P < 0.001.

Journal: Physiological Research

Article Title: Protection of Toll-Like Receptor 9 Against Lipopolysaccharide-Induced Inflammation and Oxidative Stress of Pulmonary Epithelial Cells via MyD88-Mediated Pathways

doi: 10.33549/physiolres.934741

Figure Lengend Snippet: Effect of TLR9 on LPS-induced apoptosis in A549 and Beas-2B cells. Flow cytometry was used to measure the percentage of apoptotic A549 (A) and Beas-2B (B) cells. Expression of apoptosis-related proteins caspase 8 and Bcl-2 in A549 (C) and Beas-2B (D) cells. Data represent mean ± SD (n = 3). ***P < 0.001.

Article Snippet: Plasmid and transfection The TLR9 overexpression vector pcDNA3.1 HISA (TLR9 sequence: F: 5′-CGGAATTCCGATGGGTTTCTGCCGCAGC-3′, R: 5′-CCCTCGAGGGCTATT-CGGCCGTGGGTCCC-3′) and interference vector pSIREN (shRNA1 sequence: 5′-CCTACAACCGCATCGTCAAAC-3′, shRNA2 sequence: 5′-GCTGCCCAAATCCCTCATATC-3′, shRNA3 sequence: 5′-GCTAGACCTGTCCCACAATAA-3′) were purchased from Addgene (Cambridge, MA, USA).

Techniques: Flow Cytometry, Expressing

Effect of TLR9 on the protein expression in LPS-challenged A549 and Beas-2B cells. The protein expression of MyD88, NF-κB, p-NF-κB, and AP-1 in A549 (A) and Beas-2B (B) cells. The protein expression of p-ERK1/2, p-JNK, and p-p38MAPK in A549 (C) and Beas-2B (D) cells. Data represent mean ± SD (n = 3). ***P < 0.001.

Journal: Physiological Research

Article Title: Protection of Toll-Like Receptor 9 Against Lipopolysaccharide-Induced Inflammation and Oxidative Stress of Pulmonary Epithelial Cells via MyD88-Mediated Pathways

doi: 10.33549/physiolres.934741

Figure Lengend Snippet: Effect of TLR9 on the protein expression in LPS-challenged A549 and Beas-2B cells. The protein expression of MyD88, NF-κB, p-NF-κB, and AP-1 in A549 (A) and Beas-2B (B) cells. The protein expression of p-ERK1/2, p-JNK, and p-p38MAPK in A549 (C) and Beas-2B (D) cells. Data represent mean ± SD (n = 3). ***P < 0.001.

Article Snippet: Plasmid and transfection The TLR9 overexpression vector pcDNA3.1 HISA (TLR9 sequence: F: 5′-CGGAATTCCGATGGGTTTCTGCCGCAGC-3′, R: 5′-CCCTCGAGGGCTATT-CGGCCGTGGGTCCC-3′) and interference vector pSIREN (shRNA1 sequence: 5′-CCTACAACCGCATCGTCAAAC-3′, shRNA2 sequence: 5′-GCTGCCCAAATCCCTCATATC-3′, shRNA3 sequence: 5′-GCTAGACCTGTCCCACAATAA-3′) were purchased from Addgene (Cambridge, MA, USA).

Techniques: Expressing

Results of replicate experiments for Biochemical testing in A549 cells

Journal: Physiological Research

Article Title: Protection of Toll-Like Receptor 9 Against Lipopolysaccharide-Induced Inflammation and Oxidative Stress of Pulmonary Epithelial Cells via MyD88-Mediated Pathways

doi: 10.33549/physiolres.934741

Figure Lengend Snippet: Results of replicate experiments for Biochemical testing in A549 cells

Article Snippet: Plasmid and transfection The TLR9 overexpression vector pcDNA3.1 HISA (TLR9 sequence: F: 5′-CGGAATTCCGATGGGTTTCTGCCGCAGC-3′, R: 5′-CCCTCGAGGGCTATT-CGGCCGTGGGTCCC-3′) and interference vector pSIREN (shRNA1 sequence: 5′-CCTACAACCGCATCGTCAAAC-3′, shRNA2 sequence: 5′-GCTGCCCAAATCCCTCATATC-3′, shRNA3 sequence: 5′-GCTAGACCTGTCCCACAATAA-3′) were purchased from Addgene (Cambridge, MA, USA).

Techniques: